RT-PCR Heteroduplex Analysis Permits Differentiation of Transgene and Host Gene Expression in a Transgenic Animal Model
In transgenic animal models, the conservation of DNA sequences between the transgene and the host wild-type gene can complicate the evaluation of the expression of each gene.The potential for gene silencing may complicate matters further.Here we report the use of RT-PCR heteroduplex analysis to differentiate the expression of a transgene and its em